Single-cell immune profiling reveals distinct immune response in asymptomatic COVID-19 patients

Abstract While some individuals infected by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) present mild-to-severe disease, many SARS-CoV-2-infected are asymptomatic. We sought to identify the distinction of immune response between asymptomatic and moderate patients. performed single-cell transcriptome T-cell/B-cell receptor (TCR/BCR) sequencing in 37 longitudinal collected peripheral blood mononuclear cell samples from asymptomatic, moderate, patients with healthy controls. Asymptomatic displayed increased CD56 bri CD16 ? natural killer (NK) cells upregulation interferon-gamma effector CD4 + CD8 T NK cells. They showed more robust TCR clonal expansion, especially cells, but lack strong BCR expansion compared Moreover, have lower interferon-stimulated genes (ISGs) expression general large interpatient variability, whereas various magnitude temporal dynamics ISGs across multiple populations than a patient disease. Our data provide evidence different signatures SARS-CoV-2 infections.